The Holland Tunnel is a major tunnel crossing beneath the Hudson River and linking the island of Manhattan in New York City on the river's east bank with the U.S. state of New Jersey on the river's west bank.

In the original 1989 video game, part of the game's second area takes place near here, before the turtles dive into the Hudson River.

In the IDW continuity's Casey & April issue #1, April O'Neil and Casey Jones drive April's van through the Holland Tunnel as part of the first leg of their road trip to the American Southwest.